Phys. Rev. Lett. 103, 198101 (2009) [4 pages]Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast CellReceived 1 June 2009; revised 29 July 2009; published 5 November 2009 We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 °C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics. © 2009 The American Physical Society URL:
http://link.aps.org/doi/10.1103/PhysRevLett.103.198101
DOI:
10.1103/PhysRevLett.103.198101
PACS:
87.59.−e, 42.30.Rx, 61.05.cp, 87.16.−b
See AlsoSee Also: Enju Lima, Lutz Wiegart, Petra Pernot, Malcolm Howells, Joanna Timmins, Federico Zontone, and Anders Madsen, Cryogenic X-Ray Diffraction Microscopy for Biological Samples, Phys. Rev. Lett. 103, 198102 (2009). |
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