Phys. Rev. Lett. 103, 198102 (2009) [4 pages]Cryogenic X-Ray Diffraction Microscopy for Biological SamplesReceived 1 June 2009; revised 14 August 2009; published 5 November 2009 X-ray diffraction microscopy (XDM) is well suited for nondestructive, high-resolution biological imaging, especially for thick samples, with the high penetration power of x rays and without limitations imposed by a lens. We developed nonvacuum, cryogenic (cryo-) XDM with hard x rays at 8 keV and report the first frozen-hydrated imaging by XDM. By preserving samples in amorphous ice, the risk of artifacts associated with dehydration or chemical fixation is avoided, ensuring the imaging condition closest to their natural state. The reconstruction shows internal structures of intact D. radiodurans bacteria in their natural contrast. © 2009 The American Physical Society URL:
http://link.aps.org/doi/10.1103/PhysRevLett.103.198102
DOI:
10.1103/PhysRevLett.103.198102
PACS:
87.59.−e, 61.05.cp
See AlsoSee Also: Xiaojing Huang, Johanna Nelson, Janos Kirz, Enju Lima, Stefano Marchesini, Huijie Miao, Aaron M. Neiman, David Shapiro, Jan Steinbrener, Andrew Stewart, Joshua J. Turner, and Chris Jacobsen, Soft X-Ray Diffraction Microscopy of a Frozen Hydrated Yeast Cell, Phys. Rev. Lett. 103, 198101 (2009). |
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