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Phys. Rev. Lett. 80, 2093–2096 (1998)

Hindered Rotational Diffusion and Rotational Jumps of Single Molecules

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T. Ha1, J. Glass1,2, Th. Enderle1,*, D. S. Chemla1,2, and S. Weiss1
1Materials Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720
2Department of Physics, University of California at Berkeley, Berkeley, California 94720

Received 19 September 1997; published in the issue dated 9 March 1998

We extended the sensitivity of fluorescence polarization anisotropy measurements to the single molecule level by studying the polarization response of single fluorophores rapidly rotating in liquid. Comparison with a simple model calculation allows us to obtain information on the hindered motion as well as on the rotational diffusion in the presence of specific molecular interactions. We also observed rotational jumps between surface-bound and unbound states and show that they are, similar to jumps to long dark states, photoactivated.

© 1998 The American Physical Society

URL:
http://link.aps.org/doi/10.1103/PhysRevLett.80.2093
DOI:
10.1103/PhysRevLett.80.2093
PACS:
33.15.Kr, 33.50.-j, 33.80.-b

*Present address: Pharma Preclinical R&D, F. Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland.

 
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