Phys. Rev. Lett. 80, 1786–1789 (1998)Shape Changes of Self-Assembled Actin Bilayer Composite MembranesReceived 2 June 1997; published in the issue dated 23 February 1998 We report the self-assembly of thin actin shells beneath the membranes of giant vesicles. Ion-carrier mediated influx of Mg2+ induces actin polymerization in the initially spherical vesicles. Buckling of the vesicles and the formation of blisters after thermally induced bilayer expansion are demonstrated. Bilayer flickering is dominated by tension generated by its coupling to the actin cortex. Quantitative flicker analysis suggests that the bilayer and the actin cortex are separated by 0.4 to 0.5μm due to undulation forces. © 1998 The American Physical Society URL:
http://link.aps.org/doi/10.1103/PhysRevLett.80.1786
DOI:
10.1103/PhysRevLett.80.1786
PACS:
87.22.Bt, 82.70.-y, 87.45.-k
|
