Phys. Rev. Lett. 83, 444–447 (1999)Imaging of Vapor Plumes Produced by Matrix Assisted Laser Desorption: A Plume Sharpening EffectReceived 31 December 1998; published in the issue dated 12 July 1999 The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components. © 1999 The American Physical Society URL:
http://link.aps.org/doi/10.1103/PhysRevLett.83.444
DOI:
10.1103/PhysRevLett.83.444
PACS:
79.20.Ds, 47.70.Nd, 82.80.Ms, 87.80.-y
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