Phys. Rev. Lett. 84, 5010–5013 (2000)Designing Protein β-Sheet Surfaces by Z-Score OptimizationReceived 27 September 1999; published in the issue dated 22 May 2000 Studies of lattice models of proteins have suggested that the appropriate energy expression for protein design may include nonthermodynamic terms to accommodate negative design concerns. One method, developed in lattice model studies, maximizes a quantity known as the “ Z-score,” which compares the lowest energy sequence whose ground state structure is the target structure to an ensemble of random sequences. Here we show that, in certain circumstances, the technique can be applied to real proteins. The resulting energy expression is used to design the β-sheet surfaces of two real proteins. We find experimentally that the designed proteins are stable and well folded, and in one case is even more thermostable than the wild type. © 2000 The American Physical Society URL:
http://link.aps.org/doi/10.1103/PhysRevLett.84.5010
DOI:
10.1103/PhysRevLett.84.5010
PACS:
87.15.Aa, 87.10.+e, 87.14.Ee, 87.15.Cc
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