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Phys. Rev. Lett. 90, 223002 (2003) [4 pages]

Photodestruction Intermediates Probed by an Adjacent Reporter Molecule

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Taekjip Ha and Jian Xu
Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, 61801, USA

Received 18 November 2001; revised 5 December 2002; published 5 June 2003

We used a fluorescence resonance energy transfer donor molecule to probe the multiple intermediates in the photoinduced destruction of an acceptor molecule. These intermediates are nonemitting but are still able to quench the fluorescence of the donor at a distance scale shorter than conventional fluorescence resonance energy transfer, suggesting novel biophysical applications.

© 2003 The American Physical Society

URL:
http://link.aps.org/doi/10.1103/PhysRevLett.90.223002
DOI:
10.1103/PhysRevLett.90.223002
PACS:
33.50.–j, 87.64.–t