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Phys. Rev. Lett. 93, 078102 (2004) [4 pages]

Anomalous Diffusion in Living Yeast Cells

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Iva Marija Tolić-Nørrelykke1,*, Emilia-Laura Munteanu1, Genevieve Thon2, Lene Oddershede1, and Kirstine Berg-Sørensen1
1The Niels Bohr Institute, Blegdamsvej 17, DK-2100 Copenhagen Ø, Denmark
2Institute of Molecular Biology, Øster Farigmagsgade 2A, DK-1353 Copenhagen K, Denmark

Received 31 July 2003; published 13 August 2004

The viscoelastic properties of the cytoplasm of living yeast cells were investigated by studying the motion of lipid granules naturally occurring in the cytoplasm. A large frequency range of observation was obtained by a combination of video-based and laser-based tracking methods. At time scales from 10-4 to 102   s, the granules typically perform subdiffusive motion with characteristics different from previous measurements in living cells. This subdiffusive behavior is thought to be due to the presence of polymer networks and membranous structures in the cytoplasm. Consistent with this hypothesis, we observe that the motion becomes less subdiffusive upon actin disruption.

© 2004 The American Physical Society

URL:
http://link.aps.org/doi/10.1103/PhysRevLett.93.078102
DOI:
10.1103/PhysRevLett.93.078102
PACS:
87.80.Cc, 05.40.Jc, 46.35.+z

*Permanent address: Rugjer Bošković Institute, Bijenička 54, HR-10000 Zagreb, Croatia.