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Phys. Rev. Lett. 94, 088102 (2005) [4 pages]

Passive or Active Fluctuations in Membranes Containing Proteins

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P. Girard1, J. Prost1,2, and P. Bassereau1,*
1PhysicoChimie Curie, UMR CNRS-Institut Curie 168, 11 rue Pierre et Marie Curie, 75231 Paris CEDEX 05, France
2ESPCI, 10 rue Vauquelin, 75231 Paris CEDEX 05, France

Received 27 July 2004; published 1 March 2005

We have experimentally investigated the effect of a transmembrane protein, the Ca2+-ATPase, on shape fluctuations of giant vesicles. By using the micropipette method, we have measured a substantial renormalization of the bending modulus due to the presence of proteins in the membrane. Moreover, we have produced the first quantitative measurement of the active force dipole associated with the amplification of the fluctuations when the proteins are activated by adenosine 5′-triphosphate (ATP).

© 2005 The American Physical Society

URL:
http://link.aps.org/doi/10.1103/PhysRevLett.94.088102
DOI:
10.1103/PhysRevLett.94.088102
PACS:
87.16.Uv, 05.40.–a, 87.15.–v, 87.68.+z

*Electronic address: patricia.bassereau@curie.fr