Phys. Rev. Lett. 99, 068101 (2007) [4 pages]Experimental Free Energy Surface Reconstruction from Single-Molecule Force Spectroscopy using Jarzynski’s Equality
We used the atomic force microscope to manipulate and unfold individual molecules of the titin I27 domain and reconstructed its free energy surface using Jarzynski’s equality. The free energy surface for both stretching and unfolding was reconstructed using an exact formula that relates the nonequilibrium work fluctuations to the molecular free energy. In addition, the unfolding free energy barrier, i.e., the activation energy, was directly obtained from experimental data for the first time. This Letter demonstrates that Jarzynski’s equality can be used to analyze nonequilibrium single-molecule experiments, and to obtain the free energy surfaces for molecular systems, including interactions for which only nonequilibrium work can be measured. This article is available under the terms of the Creative Commons Attribution 3.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. © 2007 The American Physical Society URL:
http://link.aps.org/doi/10.1103/PhysRevLett.99.068101
DOI:
10.1103/PhysRevLett.99.068101
PACS:
87.15.He, 87.14.Ee, 87.64.Dz
See AlsoComment: Raymond W. Friddle, Comment on “Experimental Free Energy Surface Reconstruction from Single-Molecule Force Spectroscopy Using Jarzynski’s Equality”, Phys. Rev. Lett. 100, 019801 (2008). Reply: N. C. Harris, Y. Song, and C.-H. Kiang, Harris, Song, and Kiang Reply:, Phys. Rev. Lett. 100, 019802 (2008). |
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